Author(s): Rucha A Patel, Meghna P. Patel, Hasumati A. Raj, Nehal Shah

Email(s): ruchajigar6114@gmail.com

DOI: 10.5958/2231-5691.2015.00003.9   

Address: Rucha A Patel1*, Meghna P. Patel1, Hasumati A. Raj1, Nehal Shah2
1Department of Quality Assurance, Shree Dhanvantry Pharmacy College, Kim, Surat, Gujarat, India
2 Dharmaj Degree of Pharmacy, Dharmaj, Anand, Gujarat, India
*Corresponding Author

Published In:   Volume - 5,      Issue - 1,     Year - 2015


ABSTRACT:
An approach of forced degradation study was successfully applied for the development of a stability-indicating high performance liquid chromatographic method for simultaneous determination of Olmesartan medoxomil and Indapamide in a formulation in the presence of its degradation products. In the present study a simple, accurate and precise reverse phase liquid chromatographic method has been developed and validated for simultaneous estimation of Olmesartan medoxomil and Indapamide in tablet dosage form. Developed Method was achieved on symmetry C18 (150 mm × 4.6 mm, 5 µ) column using a Acetonitrile: 0.02 M Na2HPO4 (45:55 v/v) mobile phase and pH 7 adjusted with ortho phosphoric acid. Isocratic elution mode at a flow rate of 1.0 ml/min at Room temperature with a load of 20µl Injection volume. The detection was carried out at 240 nm. The linearity of the proposed method was investigated in the range of 50-250 microg/mL (r2 = 0.998) for Olmesartan medoxomil and 10-50 microg/mL (r2 = 0.998) for Indapamide. The retention time of Olmesartan medoxomil and Indapamide were found to be around 4.79 min and 7.59 min respectively. The drug substances were subjected to stress conditions of acid hydrolysis, base hydrolysis, Oxidative, photolytic and thermal. The developed RP-HPLC method was validated with respect to linearity, accuracy, precision, robustness, LOD and LOQ.


Cite this article:
Rucha A Patel, Meghna P. Patel, Hasumati A. Raj, Nehal Shah. Development and Validation of Stability Indicating high performance liquid chromatographic Method for Olmesartan medoxomil and Indapamide in Tablet. Asian J. Pharm. Res. 5(1): Jan.-Mar. 2015; Page 15-23. doi: 10.5958/2231-5691.2015.00003.9


Recomonded Articles:

Author(s): Mercy Mathew, Ravikumar, Simila Madathil, Anju Govind, Narayana Swamy VB

DOI: 10.5958/2231-5691.2016.00015.0         Access: Open Access Read More

Author(s): Kasad Pinaz A, K.S. Muralikrishna

DOI:         Access: Open Access Read More

Author(s): Neha Meshram, Mithlesh Ojha, Ajay Singh, Amit Alexander, Ajazuddin, Mukesh Sharma

DOI: 10.5958/2231-5691.2015.00009.X         Access: Open Access Read More

Author(s): Prabhu Venkatesh Moodbidri, Varadaraji Dhayanithi, Ganesh Belavadi Manjunathashastry, Hari Narayan Pati , Pardhasaradhi Vasireddy

DOI: 10.5958/2231-5691.2015.00027.1         Access: Open Access Read More

Author(s): Tandel Jinal N., Pumbhadiya Dilipkumar A., Chauhan Payal P, Shah Samir K

DOI: 10.5958/2231-5691.2018.00001.1         Access: Open Access Read More

Author(s): Indrajeet S. Patil, Omkar A. Patil, Girish Chandra R. Mandake, Manoj M. Nitalikar

DOI: 10.5958/2231-5691.2018.00035.7         Access: Closed Access Read More

Author(s): Mahmoud M. Sebaiy, Abdullah A. El-Shanawany, Sobhy M. El-Adl, Lobna M. Abdel-Aziz, Hisham A. Hashem

DOI:         Access: Open Access Read More

Author(s): Goli. Venkateshwarlu, Ragya Eslavath, A.Santhosh, Gutha Suma, E.Rajeshwari, CH.Ramesh

DOI:         Access: Open Access Read More

Author(s): Sarfaraz Md., Prasad Y., Reddy S.R, Doddayya H. , Udupi R.H.

DOI:         Access: Open Access Read More

Author(s): Indu Sharma, Bharat Parashar, Hitesh Kumar Dhamija , Ritu sharma

DOI:         Access: Open Access Read More

Author(s): B. Babu Rao, P. Harish, Saikat Das, T. Ashwin Kumar, V. Raman Koundinya

DOI:         Access: Open Access Read More

Author(s): Jadhav Sameer S., Salunkhe Vijay R. , Magdum Chandrakant S.

DOI:         Access: Open Access Read More

Author(s): Jagdish Jadhav, Dharmendra Mundhada, Rajesh Mujoriya

DOI: 10.5958/2231-5691.2015.00019.2         Access: Open Access Read More

Author(s): Mohd. Yaqub Khan, Poonam Gupta, Bipin Bihari, Vikas Kumar Verma

DOI:         Access: Open Access Read More

Asian Journal of Pharmaceutical Research (AJPRes.) is an international, peer-reviewed journal, devoted to pharmaceutical sciences. AJPRes. publishes Original Research Articles, Short Communications..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231–5691 


Recent Articles




Tags