Author(s):
Aman Choudhary, Rita Saini, Shivanand M. Patil
Email(s):
aman12akc2@gmail.com
DOI:
10.52711/2231-5691.2026.00006 
Address:
Aman Choudhary*, Rita Saini, Shivanand M. Patil
Department of Pharmacy, Shree Dev Bhoomi Institute of Education Science and Technology, Pondha, Dehradun, Uttarakhand – 248007.
*Corresponding Author
Published In:
Volume - 16,
Issue - 1,
Year - 2026
ABSTRACT:
Background: The examination of plant-based substitutes is required due to the growing resistance of harmful microbes to traditional antibiotics. The growth of antibiotic-resistant bacteria presents a significant threat to public health, necessitating the development of alternative antimicrobial drugs. Zingiber officinale (ginger) has long been utilized for its therapeutic characteristics, including antibacterial activity. Purpose: The purpose of this study was to investigate the potential of Zingiber officinale as a natural antimicrobial agent by assessing the phytochemical components and antibacterial activity of rhizome extracts against Escherichia coli. Method: The rhizomes of fresh ginger were washed, dried, and then crushed into a coarse powder. Petroleum ether, chloroform, and ethanol were used in the maceration process for extraction. To find substances such flavonoids, alkaloids, phenols, terpenoids, tannins, steroids, and glycosides, the extracts underwent qualitative phytochemical screening. The agar well diffusion technique was used to evaluate the antibacterial activity at doses of 50, 100, and 150mg/ml. The standard reference was ciprofloxacin (30mg/ml). Results: In comparison to petroleum ether extracts (16mm) and chloroform (19mm), ethanol extract exhibited the strongest antibacterial activity and the richest phytochemical profile, with a maximal inhibition zone of 22mm at 150mg/ml concentration. Ethanol extract demonstrated exceptional effectiveness with a minimum inhibitory concentration (MIC) of 100mg/ml. Conclusion: Based on the results, Zingiber officinale rhizome has strong antibacterial properties, especially its ethanol extract. This demonstrates that it may be used to treat bacterial infections as a natural substitute for conventional antibiotics.
Cite this article:
Aman Choudhary, Rita Saini, Shivanand M. Patil. Extraction, Phytochemical Screening and Antibacterial Activity of Zingiber officinale Rhizome extracts against Microorganism. Asian Journal of Pharmaceutical Research. 2026; 16(1):44-0. doi: 10.52711/2231-5691.2026.00006
Cite(Electronic):
Aman Choudhary, Rita Saini, Shivanand M. Patil. Extraction, Phytochemical Screening and Antibacterial Activity of Zingiber officinale Rhizome extracts against Microorganism. Asian Journal of Pharmaceutical Research. 2026; 16(1):44-0. doi: 10.52711/2231-5691.2026.00006 Available on: https://asianjpr.com/AbstractView.aspx?PID=2026-16-1-6
REFERENCE:
1. Levita J, Mutakin M, Diantini A. A Review: Is Ginger (Zingiber officinale var. Roscoe) Potential for Future Phytomedicine? Indonesian Journal of Applied Sciences. 2018 Apr; 8(1):1–6.
2. Thakur K, Devi DrA, Kumar DrS. Formulation and evaluation of antimicrobial potential of glycerosomes containing allium sativum, Zingiber officinale extracts. Journal of Advanced Zoology. 2023 Oct; 44(S2):1264–1288.
3. BHOVİ VK. Plants based materials as the antifungal and antibacterial agents. International Journal of Plant-based Pharmaceuticals. 2022 Feb 1; 2(1):98–110.
4. Gupta S kumar, Sharma A. Medicinal properties of Zingiber officinale Roscoe - A Review. IOSR Journal of Pharmacy and Biological Sciences. 2014; 9(5):124–9.
5. Liang J, Stöppelmann F, Schoenbach J, Rigling M, Nedele AK, Zhang Y, et al. Influence of peeling on volatile and non-volatile compounds responsible for aroma, sensory, and nutrition in ginger (Zingiber officinale). Food Chemistry [Internet]. 2023 Sep; 419:136036.
6. Marwat SK, Shoaib M, Khan EA, Ullah H, et al. Phytochemistry and Bioactivities of Quranic Plant, Zanjabil-Ginger (Zingiber officinale Roscoe): A Review. American-Eurasian J Agric and Environ Sci. 2015; 15(5):707–713.
7. Stoner GD. Ginger: Is it Ready for Prime Time? Cancer Prevention Research. 2013 Apr 1; 6(4):257–62.
8. Ji K, Fang L, Zhao H, Li Q, Shi Y, Xu C, et al. Ginger Oleoresin Alleviated γ-Ray Irradiation-Induced Reactive Oxygen Species via the Nrf2 Protective Response in Human Mesenchymal Stem Cells. Oxidative Medicine and Cellular Longevity. 2017; 2017:1480294.
9. Moon, YS., Lee, HS. and Lee, SE. Inhibitory effects of three monoterpenes from ginger essential oil on growth and aflatoxin production of Aspergillus flavus and their gene regulation in aflatoxin biosynthesis. Appl Biol Chem. 2018; 61: 243–250. https://doi.org/10.1007/s13765-018-0352-x
10. Samuel Botião Nerilo, Rocha G, Tomoike C, Aparecida S, Grespan R, Martha J, et al. Antifungal properties and inhibitory effects upon aflatoxin production by Zingiber officinale essential oil inAspergillus flavus. 2016 Feb 1; 51(2): 286–92.
11. Yamamoto-Ribeiro MMG, Grespan R, Kohiyama CY, Ferreira FD, Mossini SAG, Silva EL, et al. Effect of Zingiber officinale essential oil on Fusarium verticillioides and fumonisin production. Food Chemistry. 2013 Dec; 141(3): 3147–52.
12. Rampogu S, Baek A, Gajula RG, Zeb A, Bavi RS, Kumar R, et al. Ginger (Zingiber officinale) phytochemicals—gingerenone-A and shogaol inhibit SaHPPK: molecular docking, molecular dynamics simulations and in vitro approaches. Annals of Clinical Microbiology and Antimicrobials. 2018 Apr 2; 17(1).
13. Chakotiya AS, Tanwar A, Narula A, Sharma RK. Zingiber officinale: Its antibacterial activity on Pseudomonas aeruginosa and mode of action evaluated by flow cytometry. Microbial Pathogenesis. 2017 Jun; 107: 254–60.
14. Hasan S, Danishuddin M, Khan AU. Inhibitory effect of zingiber officinale towards Streptococcus mutans virulence and caries development: in vitro and in vivo studies. BMC Microbiology. 2015; 15(1): 1.
15. Chang JS, Wang KC, Yeh CF, Shieh DE, Chiang LC. Fresh ginger (Zingiber officinale) has anti-viral activity against human respiratory syncytial virus in human respiratory tract cell lines. Journal of Ethnopharmacology. 2013 Jan; 145(1): 146–51.
16. Supu RD, Diantini A, Levita J. Red ginger (Zingiber officinale var. rubrum): its chemical constituents, pharmacological activities and safety. fitofarmaka: Jurnal Ilmiah Farmasi. 2019 May 30; 8(1):23–9.
17. Pan SY, Litscher G, Gao SH, Zhou SF, Yu ZL, Chen HQ, et al. Historical Perspective of Traditional Indigenous Medical Practices: The Current Renaissance and Conservation of Herbal Resources. Evidence-Based Complementary and Alternative Medicine. 2014 Apr 27; 2014(525340): 1–20.
18. Shiva S, Anjana P, Navami MV, Sreedhar KM, Sreekanth KM, Sivasubramanian G. Bioactive potential enhancement of Ginger (Zingiber officinale) through ball-mill assisted micronization. Food Chemistry Advances. 2025 March; 7(100970): 100970.
19. Syarif RA, Faradiba F, Alyanti TK, Savitri TA. GC-MS Analysis of Ginger Rhizome with Various Extraction Methods. Jurnal Fitofarmaka Indonesia. 2024 Dec; 11(3): 107–114.
20. Bitwell C, Indra SS, Luke C, Kakoma MK. A review of modern and conventional extraction techniques and their applications for extracting phytochemicals from plants. Scientific African. 2023 Mar 1; 19:e01585
21. Nguyen ST, Vo PH, Nguyen TD, Do NM, Le BH, Dinh DT, et al. Ethanol extract of Ginger Zingiber officinale Roscoe by Soxhlet method induces apoptosis in human hepatocellular carcinoma cell line. Biomedical Research and Therapy. 2019 Nov 22; 6(11): 3433–42.
22. An K, Zhao D, Wang Z, Wu J, Xu Y, Xiao G. Comparison of different drying methods on Chinese ginger (Zingiber officinale Roscoe): Changes in volatiles, chemical profile, antioxidant properties, and microstructure. Food Chemistry. 2016 Apr; 197: 1292–300.
23. Lucky E, Igbinosa OE, Jonahan I. Antimicrobial Activity of Zingiber officinale Against Multidrug Resistant Microbial Isolates. Health Sciences Research. 2017 Nov; 4(6): 76–81.
24. Bhargava S, Dhabhai K, Batra A, Sharma A, Malhotra B. Zingiber Officinale : Chemical and phytochemical screening and evaluation of its antimicrobial activities. Journal of Chemical and Pharmaceutical Research. 2012; 4(1): 360–364.
25. Pandey N, Walia A. Datura stramonium leaves: A Potential Element of Anti –Microbial Activity. Universities Journal of Phytochemistry and Ayurvedic Heights. 2024 Dec 7; II(37).
26. Sabir S, Anjum AA, Ijaz T, Ali MA, Rehman MU, Nawaz M. Isolation and antibiotic susceptibility of E. coli from urinary tract infections in a tertiary care hospital. Pakistan Journal of Medical Sciences. 1969 Dec 31; 30(2).
27. Vranic S, Uzunovic A. Antimicrobial Resistance of Escherichia Coli Strains Isolated from Urine at Outpatient Population: a Single Laboratory Experience. Materia Socio Medica. 2016; 28(2): 121.
28. Russo TA, Carlino UB, Mong A, Jodush ST. Identification of Genes in an Extraintestinal Isolate of Escherichia coli with Increased Expression after Exposure to Human Urine. Infection and Immunity. 1999 Oct 1; 67(10): 5306–14.
29. Prevalence and Antibiotic Susceptibility Pattern of Escherichia Coli Isolated from Urine Samples of Urinary Tract Infection Patients. ARC Journal of Urology. 2019; 4(1).
30. Mainasara MM, Aliero BL, et.al. Phytochemical and Antibacterial Properties of Root and Leaf Extracts of Calotropis procera. Nigerian Journal of Basic and Applied Science. 2012 Mar; 20(1): 1–6.
31. De S, Pramanik A, Das AK, Paul S, Jana S, Pramanik P. Isolation and characterization of Lactobacillus spp. from curd and its pharmacological application in probiotic chocolate. The Journal of Phytopharmacology. 2017 Dec; 6(6): 335–339.
32. Athanassiadis B, Abbott P, George N, Walsh L. Anin vitrostudy of the antimicrobial activity of some endodontic medicaments and their bases using an agar well diffusion assay. Australian Dental Journal. 2009 Jun; 54(2): 141–6.
33. Nyamath S, Karthikeyan B. In vitro antibacterial activity of lemongrass (Cymbopogon citratus) leaves extract by agar well method. Journal of Pharmacognosy and Phytochemistry. 2018 May; 7(3): 1185–1188.