Author(s): Vijayakrishnan S R, Deepa Parvathi V, R Sumitha

Email(s): sumithamadhu79@gmail.com

DOI: 10.52711/2231-5691.2023.00044   

Address: Vijayakrishnan S R, Deepa Parvathi V, R Sumitha*
Department of Biomedical Sciences, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai - 116.
*Corresponding Author

Published In:   Volume - 13,      Issue - 4,     Year - 2023


ABSTRACT:
Environmental pollution due to accumulation of synthetic polymers namely plastics is a growing concern which threatens the terrestrial marine flora and fauna. Traditional methods of plastic disposal include incineration and disposal into landfills or water bodies. Incineration of polyethylene, polystyrene leads to emission of a large amount of carbon monoxide which is toxic if inhaled and also a potent greenhouse gas. Degradation of plastic by microorganisms is an efficient and eco-friendly method employed for rapid rate of disintegration. The biofilm present in the contaminated soil survives by adapting to harsh environment by secreting hydrolysing enzymes which are potent in degradation of the accumulated plastics. The present study deals with the isolation, characterisation and evaluation of plastic degrading properties of microorganisms isolated from various soil samples collected from Chennai district. Soil samples were collected aseptically from various locations & isolated by standard plate count method. The isolated organisms were identified by staining methods and characterized by phylogenetic analysis. The organism Pseudomonas aeruginosa and Streptomyces fulvissimus were further subjected to plastic degradation testing. The present study demonstrates the ability of Pseudomonas aeruginosa and Streptomyces fulvissimus to degrade polyethylene sheets.


Cite this article:
Vijayakrishnan S R, Deepa Parvathi V, R Sumitha. Isolation, Characterisation and Evaluation of Plastic Degrading Properties of Soil Biofilms Collected from Chennai District. Asian Journal of Pharmaceutical Research. 2023; 13(4):237-3. doi: 10.52711/2231-5691.2023.00044

Cite(Electronic):
Vijayakrishnan S R, Deepa Parvathi V, R Sumitha. Isolation, Characterisation and Evaluation of Plastic Degrading Properties of Soil Biofilms Collected from Chennai District. Asian Journal of Pharmaceutical Research. 2023; 13(4):237-3. doi: 10.52711/2231-5691.2023.00044   Available on: https://asianjpr.com/AbstractView.aspx?PID=2023-13-4-5


REFERENCES:
1.    Kamble Asmita, Tanwar Shubham Singh and Shanbhag Tejashree. Isolation of Plastic Degrading Microorganisms from Soil Samples Collected at Various Locations in Mumbai, India. IRJEvS. 2015;   4(3): 77-85.
2.    Dominik Danso, Jennifer Chow, Wolfgang R. Streit. Plastics: Environmental and Biotechnological Perspectives on Microbial Degradation. ASM. 2019; 85(19): 1-14.
3.    Christabel Ndahebwa Muhonja, et al. Biodegradability of polyethylene by bacteria and fungi from Dandora dumpsite Nairobi, Kenya. PLoS One. 2018; 13(7): 1-17.
4.    N. Priyanka, T. Archana. Biodegradability of Polythene and Plastic by the Help of Microorganism: A Way for a Brighter Future. J. Environ. Analytic. Toxicol. 2011; 1(4).
5.    M. Ariba Begum, B. Varalakshmi and K. Umamaheswari. Biodegradation of Polythene Bag using Bacteria Isolated from Soil. IJCMAS. 2015; 4(11): 674-680.
6.    Jill E. Clarridge. Impact of 16S rRNA Gene Sequence Analysis for Identification of Bacteria on Clinical Microbiology and Infectious Diseases. Clin Microbiol Rev. 2004; 17(4): 840–862.
7.    Deepika, S., Jaya Madhuri, R. Biodegradation Of Low Density Polyethylene By Microorganisms from Garbage soil. J.Exp. Biol.Agriculture. Sci. 2015; 3(1): 1521.
8.    Godleads Adams, et al. Bioremediation, Bio stimulation and Bioaugmentation: A Review. IJEBB. 2015; l3: 28-39.
9.    Jyoti Kaushal, Madhu Khatri, Shailendra Kumar Arya. Recent insight into enzymatic degradation of plastics prevalent in the environment: A mini - review. Cleaner Engineering and Technology. 2022; 2: 1-5.  
10.    Wilkes, R.A. and Aristilde L. Degradation and metabolism of synthetic plastics and associated products by Pseudomonas sp. capabilities and challenges. J Appl Microbiol. 2017; 123: 582-593.
11.    Tribedi, P. and Sil, A.K. Bioaugmentation of polyethylene succinate-contaminated soil with Pseudomonas sp. AKS2 results in increased microbial activity and better polymer degradation. Env Sci Pollut Res. 2013; 20; 1318–1326.
12.    Yoon, M., Jeong Jeon, H. and Nam Kim, M. Biodegradation of polyethylene by a soil bacterium and AlkB cloned recombinant cell. J Bioremediat Biodegrad. 2012; 3: 1–8.
13.    Belhaj A, Desnoues N, Elmerich C. Alkane biodegradation in Pseudomonas aeruginosa strains isolated from a polluted zone: identification of alkB and alkB-related genes. Res Microbial. 2002; 153: 339-344.
14.    Kathiresan K., Polythene and Plastics-degrading microbes from the mangrove soil, Rev. Biol. Trop. 2003; 51(3):  629-634.
15.    Arutchelvi, J, et al. Biodegradation of Polyethylene and Polypropylene. Indian J. Biotechnol. 2008.7: 922.
16.    Kyaw, B., et al. Biodegradation of Low Density Polythene (LDPE) by Pseudomonas species. Indian J. Microbiol. 2012; 52(3):411419.
17.    M A El-Nakeeb, H A Lechevalier, Selective isolation of aerobic Actinomycetes. Appl Microbiol. 1963;  11(2):75-7.
18.    Pooja Sharma.Role and significance of biofilm-forming microbes in phytoremediation -A review. Environmental Technology & Innovation. 2021; 25: 1-7.
19.    Annette E. LaBauve and Matthew J. Wargo, Growth and Laboratory Maintenance of Pseudomonas aeruginosa.  Curr Protoc Microbiol. 2012; 6(1): 1-8.
20.    Vatseldutt S., Anbuselvi.Isolation and characterization of polythene degrading bacteria from polythene dumped garbage.Int. J. Pharm. Sci. 2014. 25(2): 205-206.
21.    Usha R., Sangeetha T., Palaniswamy M. Screening of polythene degrading microorganisms from garbage soil. LibyanAgricult. Res. Center J. Int. 2012; 2: 200-204.
22.    Lee B., Pometto A.L., Fratzke A., Bailey T. B. Biodegradation of degradable plastic polyethylene by phanerochaete and streptomyces species. Appl. Environ.  Microbiol. 1991; 57: 678-685.
23.    Prabhat S. et al. Studies on Isolation and Identification of Active Microorganisms during Degradation of Polyethylene / Starch Film, Int. Res. J. Env. Sci. 2013; 2(9): 83-85.
24.    Myint B, et al. Biodegradation of Low-Density Polythene (LDPE) by Pseudomonas Species. Indian J Microbiol. 2012; 3: 411–419.
25.    Mahalakshmi V, Siddiq A, Andrew SN. Analysis of Polyethylene Degrading Potentials of Microorganisms Isolated from Compost Soil. International Journal of Pharmaceutical & Biological Archives. 2012; 3: 1190–1196.

Recomonded Articles:

Author(s): Ashok Thulluru, Nawaz Mahammed, C. Madhavi, K. Nandini, S. Sirisha, D. Spandana

DOI: 10.5958/2231-5691.2019.00016.9         Access: Open Access Read More

Author(s): S.K. Purohit, R. Solanki, V. Mathur, M. Mathur

DOI:         Access: Open Access Read More

Author(s): Rutuja S. Shah, Rutuja R. Shah, Manoj M. Nitalikar, Chandrakant S. Magdum

DOI: 10.5958/2231-5691.2017.00024.7         Access: Open Access Read More

Author(s): Mukul Ahmed, Ravikumar, Narayanaswamy VB, Injamamul Haque, Mohibul Hoque

DOI: 10.5958/2231-5691.2016.00020.4         Access: Open Access Read More

Author(s): Dasari Nirmala, Swapna Nandhini, M. Sudhakar

DOI: 10.5958/2231-5691.2016.00012.5         Access: Open Access Read More

Author(s): Injamamul Haque, Ravikumar, Narayanaswamy VB, Mohibul Hoque

DOI: 10.5958/2231-5691.2016.00023.X         Access: Open Access Read More

Author(s): Girish Pai K., Vamshi Krishna T., Lalit Kumar, M. Sreenivasa Reddy, G. Shreenidhi, A. Praneeth Reddy

DOI:         Access: Open Access Read More

Author(s): K. Vijaya Sri, R. Harish Kumar Reddy , R. Mahesh

DOI:         Access: Open Access Read More

Author(s): Gamal A. A-Ameri, Abdu M. Alkolaibe, Ahmed M. Al-kadassy, Mawhoob N. Alkadasi, Abdubaset A. Zaide

DOI: 10.5958/2231-5691.2015.00001.5         Access: Open Access Read More

Author(s): Prakash Goudanavar, Doddayya Hiremath, Spandana D. , S. R. Reddy

DOI:         Access: Open Access Read More

Author(s): Snehal B. Kulkarni, M. M. Bari,S. D. Barhate, Ashutosh Tripath

DOI: 10.5958/2231-5691.2019.00003.0         Access: Open Access Read More

Author(s): Sugiyartono, Tutiek Purwanti, Isnaeni, Dhenok Reso Fenda Asega

DOI:         Access: Open Access Read More

Author(s): Mamatha Kola, Subharna Mallik, Pranusha Palle, Pallavi Kamarapu

DOI:         Access: Open Access Read More

Author(s): S. Sathish Kumar, G. Melchias, P. Ravikumar, R. Chandrasekar, P. Kumaravel

DOI:         Access: Open Access Read More

Author(s): S. A. Mohite, R. R. Shah, N. R. Patel

DOI: 10.5958/2231-5691.2018.00004.7         Access: Open Access Read More

Author(s): Prathibha Suvarna, Ravi Kumar, Yamunappa, Pooja Shetty, Narayana Swamy V.B.

DOI: 10.5958/2231-5691.2016.00003.4         Access: Open Access Read More

Author(s): Subhadeep Ganguly

DOI:         Access: Open Access Read More

Asian Journal of Pharmaceutical Research (AJPRes.) is an international, peer-reviewed journal, devoted to pharmaceutical sciences. AJPRes. publishes Original Research Articles, Short Communications..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231–5691 


Recent Articles




Tags