Author(s): Bhishma Sapkota, Mohan Amatya, Sujan Neupane

Email(s): sapkotabhishma@gmail.com

DOI: 10.52711/2231-5691.2024.00033   

Address: Bhishma Sapkota1,2*, Mohan Amatya1, Sujan Neupane3
1Tribhuwan University Institute of Medicine, Maharajgunj Medical Campus, Maharajgunj, Kathmandu, Nepal.
2Department of Drug Administration, Bijulibajar, New Baneshwor, Kathmandu, Nepal.
3Valley College of Technical Sciences. Nagarjun - 04, Sitapaila, Kathmandu, Nepal.
*Corresponding Author

Published In:   Volume - 14,      Issue - 3,     Year - 2024


ABSTRACT:
This study was conducted for preliminary phytochemical study of different extracts and to study antimicrobial effects of methanolic extract of Desmostachya bipinnata roots. Desmostachya bipinnata Stapf is an official drug of ayurvedic pharmacopoeia, belongs to the family of Poaceae/Gramineae. Phytochemical screening was done to identify the main groups of chemical constituents present in different extract of Desmostachya bipinnata by their colour reactions with different reagents. Four extracts viz: Petroleum, chloroform, ethyl acetate and methanolic, were subjected for carbohydrates, flavonoids, glycosides, alkaloids, saponins, proteins and amino acids using a test procedure. Preliminary antimicrobial test of D. bipinnata extract was carried out by well diffusion method. The extract was subjected for antimicrobial screening using reference standard, Gentamicin (10µg). The antimicrobial screening of the plant extract revealed the antimicrobial activity against Staphylococcus aureus (Gram positive microorganism) and Escherichia coli (Gram negative microorganism).


Cite this article:
Bhishma Sapkota, Mohan Amatya, Sujan Neupane. Phytochemical Screening and Evaluation of Antimicrobial Activity of Desmostachya bipinnata Roots. Asian Journal of Pharmaceutical Research. 2024; 14(3):206-2. doi: 10.52711/2231-5691.2024.00033

Cite(Electronic):
Bhishma Sapkota, Mohan Amatya, Sujan Neupane. Phytochemical Screening and Evaluation of Antimicrobial Activity of Desmostachya bipinnata Roots. Asian Journal of Pharmaceutical Research. 2024; 14(3):206-2. doi: 10.52711/2231-5691.2024.00033   Available on: https://asianjpr.com/AbstractView.aspx?PID=2024-14-3-2


REFERENCES:
1.    C. Katiyar, A. Gupta, S. Kanjilal, S. Katiyar, Drug discovery from plant sources: An integrated approach. AYU. 2012; 33(1): 10. DOI: 10.4103/0974-8520.100295.
2.    Shakya, Arvind and Correspondence, Arvind. Medicinal plants: Future source of new drugs. International Journal of Herbal Medicines. 2016; 4(4): 59-64. 10.13140/RG.2.1.1395.6085.
3.    C.-T. Che, V. George, T.P. Ijinu, P. Pushpangadan, K. Andrae-Marobela, Chapter 2 - Traditional Medicine, Editor(s): Simone Badal, Rupika Delgoda, Pharmacognosy, Academic Press, 2017; 15-30, ISBN 9780128021040, https://doi.org/10.1016/B978-0-12-802104-0.00002-0.
4.    Mbutho, N. P., Gqaleni, N., and Korporaal, C. M. Traditional complementary and alternative medicine: knowledge, attitudes and practices of health care workers in HIV and AIDS clinics in Durban hospitals: AJTCAM 2012; 9(3 Suppl): 64–72. https://doi.org/10.4314/ajtcam.v9i3s.8
5.    Kolhe Rohini C., Chaudhari Rajesh Y. A Review on Phytopharmacological Profile of Traditionally used medicinal plant Parkia biglandulosa (Mimosaceae). Asian J. Pharm. Res. 2020; 10(1): 34-38.
6.    Kunwar, R. M., Shrestha, K. P., and Bussmann, R. W. (2010). Traditional herbal medicine in far-west Nepal: a pharmacological appraisal. Journal of Ethnobiology and Ethnomedicine, 6, 35. https://doi.org/10.1186/1746-4269-6-35.
7.    Maurice M Iwu, Chapter 25 - Ethnobotanical approach to pharmaceutical drug discovery: strengths and limitations, Editor(s): Maurice M Iwu, Jacqueline C Wootton, Advances in Phytomedicine, Elsevier, 2002; Volume 1: 309-20, ISSN 1572-557X, ISBN 9780444508522, https://doi.org/10.1016/S1572-557X(02)80034-4.
8.    Netsanet Gonfa, Dereje Tulu, Kitessa Hundera and Dasalegn Raga | Fatih Yildiz (Reviewing editor) (2020) Ethnobotanical study of medicinal plants, its utilization, and conservation by indigenous people of Gera district, Ethiopia, Cogent Food and Agriculture, 6: 1, DOI: 10.1080/23311932.2020.1852716
9.    Jalali A, Raesi Vanani A, Shirani M. Ethnobotanical Approaches of Traditional Medicinal Plants Used in the Management of Asthma in Iran. Jundishapur J Nat Pharm Prod., 2020; 15(1): e62269. https://doi.org/10.5812/jjnpp.62269.
10.    Kunwar, Ripu M., Subedi, Bhishma P., Baral, Sushim R., Maraseni, Tek, LeBoa, Chris, Adhikari, Yagya P. and Bussmann, Rainer W.. 2020. "Ethnobotany of the Himalayas: The Nepal, Bhutanese, and Tibetan Himalayas." Springer. pp. 65-103.
11.    Tripti Jain, Darshan Dubey, Vishal Jain, Kamlesh Dashora. Regulatory Status of Traditional Medicines in Different Countries: An Overview. Research J. Pharm. and Tech. 2011; 4(7): 1007-1015.
12.    Luitel, D.R., Rokaya, M.B., Timsina, B. et al. Medicinal plants used by the Tamang community in the Makawanpur district of central Nepal. J Ethnobiology Ethnomedicine. 2014; 10(5). https://doi.org/10.1186/1746-4269-10-5.
13.    Chaudhary RP, Bhattarai SH, Basnet G, Bhatta KP, Uprety Y, Bhatta LD, Kotru R, Oli BN, Sharma LN, Khanal S, Sharma UR. Traditional practice and knowledge of indigenous and local communities in Kailash Sacred Landscape, Nepal. ICIMOD Working Paper. 2017(2017/1).
14.    Bechan, R., and Prasad, K.D. (2011). Present Status of Traditional Healthcare System In Nepal.
15.    Fabricant, D.S., and Farnsworth, N.R. The value of plants used in traditional medicine for drug discovery. Environmental Health Perspectives. 2001; 109: 69 - 75.
16.    Golla, U., and Bhimathati, S. S. (2014). Evaluation of antioxidant and DNA damage protection activity of the hydroalcoholic extract of Desmostachya bipinnata L. Stapf. TheScientificWorldJournal, 2014, 215084. https://doi.org/10.1155/2014/215084.
17.    Harish Gupta, Girendra Kumar Gautam. Biological Efficacy of Desmostachya bipinnata Grass against Allergy and Hypersensitivity using different Experimental Models. Research Journal of Pharmacy and Technology. 2023; 16(10): 4543-8.
18.    Tiwari P, Phytochemical Screening and Extraction: A Review, Internationale Pharmaceutica Sciencia. 2011; 1(1): 98-106.
19.    Otimenyin, Sunday O. Uguru, M.O. Ogbonna, A. Antimicrobial and Hypoglycemic Effects of Momordica Balsamina. Linn. Journal of Natural Product. 2008; 1: 03-09.
20.    Sahiti K, Raji P, Bennett Rohan, Divya Kumar M, Antony V. Samrot. In vitro Bioactivity Screening of Desmostachya bipinnata. Research J. Pharm. and Tech. 2016; 9(4): 361-364.
21.    T. Jeyanthi, P. Subramanian, P. Kumaravel. A Comparative Analysis of Antibacterial Activity of Withania somnifera Root Extract with Commercial Antibiotics. Asian J. Pharm. Res. 2013; 3(2): 98-102.
22.    T.V. Yuvaraj, S. Hurmath Unnissa, N.S. Surendiran, M. Azeez Ur rahman, V. Binumon. Synthesis and Antimicrobial Screening of Some Novel Cinnoline Derivatives. Asian J. Research Chem. 2010; 3(4): 853-858
23.    A. W. Bauer, W. M. M. Kirby, J. C. Sherris, M. Turck, Antibiotic Susceptibility Testing by a Standardized Single Disk Method, American Journal of Clinical Pathology. 1966; 45(4): 493–496, https://doi.org/10.1093/ajcp/45.4_ts.493.
24.    L. Yoga Latha, S. Sasidharan, Z. Zuraini, S. Suryani, L. Shirley and S. Sangetha () Antibacterial Activity and Toxicity of Psophocarpus tetragonolobus., Pharmaceutical Biology. 2007; 45(10: 31-36, DOI: 10.1080/13880200601026317.
25.    Moshi, M. J., Innocent, E., Magadula, J. J., Otieno, D. F., Weisheit, A., Mbabazi, P. K., and Nondo, R. S. Brine shrimp toxicity of some plants used as traditional medicines in Kagera Region, north western Tanzania. Tanzania Journal of Health Research. 2010; 12(1): 63–67. https://doi.org/10.4314/ thrb.v12i1.56287.
26.    Jyoti Sahu, Pushpendra Kumar Patel, Balkrishna Dubey. Effects of Methanolic Extracts of Quisqualis indica (Aerial Parts) on Passive Smoking Induced Hyperlipidemia in Rats. Asian J. Pharm. Tech. 2013; 3(1): 26-29.
27.    Luz Angélica Avila-Villa, Marcel Martínez-Porchas, Teresa Gollas-Galván, José A. López-Elías, Lauro Mercado, Álvaro Murguia-López, Fernando Mendoza-Cano, Jorge Hernández-López, Evaluation of different microalgae species and Artemia (Artemia franciscana) as possible vectors of necrotizing hepatopancreatitis bacteria, Aquaculture. 2011; 318(3–4): 273-276, ISSN 0044-8486, https://doi.org/10.1016/ j.aquaculture.2011.05.043.
28.    Ajoy, G., and Padma, C.M. Brine Shrimp Cytotoxic Activity of 50% Alcoholic Extract of Croton Bonplandianum Baill. Asian Journal of Pharmaceutical and Clinical Research. 2013; 6: 40-41.
29.    Olowa, L.F. and Nuñeza, O.M. Brine Shrimp Lethality Assay of the Ethanolic Extracts of Three Selected Species of Medicinal Plants from Iligan City. International Research Journal of Biological Sciences. 2013; 2: 74-77.
30.    D. Benito Johnson, Neethu. P. Charles, Banshongdor H Mawlieh, Timai Passah, V. Venkatanarayanan. Evaluation of Anti-Oxidant and Hepatoprotective activity of Desmostachya bipinnata Leaf Extracts by Various Hepatotoxin Induced Albino Rat Models. Res. J. Pharmacognosy and Phytochem. 2016; 8(3): 109-115.
31.    Sanjay R Patel, Ashok P Suthar, Anand M Shah, Hitesh V Hirpara, Vishal D Joshi, Mayur V Katheria. Antimicrobial Activity of Methanolic Extracts of Mucuna pruriens, Semecarpus anacardium, Anethum graveolens by Agar Disc Diffusion Method. Research J. Pharm. and Tech. 2010; 3(1): 165-167.
32.    Kokati Vankata Bhaskara Rao, Minki Munjal, Amie Patnayak, Loganathan Karthik, Gaurav Kumar. Phytochemical Composition, Antioxidant, Antimicrobial and Cytotoxic Potential of Methanolic Extracts of Adhatoda vasica (Acanthaceae). Research J. Pharm. and Tech. 2013; 6(9): 1004-1009.

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