Author(s): Ajay I. Patel, Darshita R. Vyas, Amitkumar J. Vyas, Ashok B. Patel, Ashvin V. Dudhrejiya, Urvi J. Chotaliya

Email(s): aipvk84@gmail.com , darshita.vyas227@gmail.com , amitvyas77@gmail.com , abp2038@gmail.com , ashvinvd@gmail.com , Urvi_jc@yahoo.com

DOI: 10.52711/2231-5691.2023.00046   

Address: Ajay I. Patel1, Darshita R. Vyas2*, Amitkumar J. Vyas3, Ashok B. Patel4, Ashvin V. Dudhrejiya5, Urvi J. Chotaliya6
1B. K. Mody Government Pharmacy College, Polytechnic Campus, Rajkot, Gujarat, India. Postal Code: 360003.
2B. K. Mody Government Pharmacy College, Polytechnic Campus, Rajkot, Gujarat, India. Postal Code: 360003.
3B. K. Mody Government Pharmacy College, Polytechnic Campus, Rajkot, Gujarat, India. Postal Code: 360003.
4Government Pharmacy College, Gandhinagar, Gujarat, India. Postal Code: 382027.
5B. K. Mody Government Pharmacy College, Polytechnic Campus, Rajkot, Gujarat, India. Postal Code: 360003.
6B. K. Mody Government Pharmacy College, Polytechnic Campus, Rajkot, Gujarat, India. Postal Code: 360003.
*Corresponding Author

Published In:   Volume - 13,      Issue - 4,     Year - 2023


ABSTRACT:
Proteomics is the study of proteins and involves the applications of technologies for the identification and quantification of overall proteins present content of a cell, tissue or an organism. Proteomics is developing fast due to the growing number of proteins obtained by recombinant DNA technology. Proteomics-based technologies are utilized in various capacities for different research settings such as the detection of various diagnostic markers, candidates for vaccine production, understanding pathogenicity mechanisms and interpretation of functional protein pathways in different diseases. Protein analysis employs various techniques and methods, which helps to study the proteomics. While the potential is great, many challenges and issues remain to be solved such as mining and integration of the proteomics. Over the last decade, technological advances in proteomics have driven or increased the capability of proteomics discovery. This review efforts to describe various technologies for the protein analysis, challenges and recent advances employed in the field of proteomics.


Cite this article:
Ajay I. Patel, Darshita R. Vyas, Amitkumar J. Vyas, Ashok B. Patel, Ashvin V. Dudhrejiya, Urvi J. Chotaliya. Proteomics: Technology, Challenges and Advances. Asian Journal of Pharmaceutical Research. 2023; 13(4):249-5. doi: 10.52711/2231-5691.2023.00046

Cite(Electronic):
Ajay I. Patel, Darshita R. Vyas, Amitkumar J. Vyas, Ashok B. Patel, Ashvin V. Dudhrejiya, Urvi J. Chotaliya. Proteomics: Technology, Challenges and Advances. Asian Journal of Pharmaceutical Research. 2023; 13(4):249-5. doi: 10.52711/2231-5691.2023.00046   Available on: https://asianjpr.com/AbstractView.aspx?PID=2023-13-4-7


REFERENCE:
1.    Nawsheen B. Protein Analysis: Key To The Future. Biotechniques. 2018; 64:197-201. doi:10.4155/btn-2018-0055
2.    Vittaladevaram V. Life of Proteomics: Technologies, Challenges, Viewpoints and Future. Journal of Proteomics & Bioinformatics. 2021; 14(4):1-5.
3.    Bilal A, Basit M, Nisar MA, Khurshid M, Rasool MH. Proteomics: Technologies and Their Applications. Journal of Chromatographic Science. 2017;55(2):182–196. doi: 10.1093/chromsci/bmw167
4.    Liu S, Li Z, Yua B, Wang S, Shen Y, Cong H. Recent Advances on protein separation and purification methods. Advances in Colloid and Interface Science. 2020;284.
5.    Bae SH, Harris AG, Hains PG. Strategies for the enrichment and identification of basic proteins in proteome projects. Proteomics. 2003; 3(5):569–579.
6.    Schulz TC, Swistowska AM, Liu Y. A large-scale proteomic analysis of human embryonic stem cells. BMC Genomics. 2007;8.
7.    Goshima N, Kawamura Y, Fukumoto A. Human protein factory for converting the transcriptome into an in vitro-expressed proteome. Nature Methods. 2008;5(12):1011–1017.
8.    Jones RB, Gordus A, Krall JA, MacBeath G. A quantitative protein interaction network for the ErbB receptors using protein microarrays. Nature. 2006;439(7073):168–174.
9.    Kingsmore SF and Patel DD. Multiplexed protein profiling on antibody-based microarrays by rolling circle amplification. Current Opinion in Biotechnology. 2003;14(1):74–81.
10.    Yao X, Freas A, Ramirez J, Demirev PA, Fenselau C. Proteolytic 18O labeling for comparative proteomics: model studies with two serotypes of adenovirus. Analytical Chemistry. 2001;73(13):2836–2842.
11.    Vercauteren FG, Arckens L, Quirion R. Applications and current challenges of proteomic approaches, focusing on two-dimensional electrophoresis. Amino Acids. 2008;33(3):405–414.
12.    Berggren K, Chernokalskaya E, Steinberg TH. Background-free, high sensitivity staining of proteins in one- and two-dimensional sodium dodecyl sulfatepolyacrylamide gels using a luminescent ruthenium complex. Electrophoresis. 2008;21(12):2509–2521.
13.    Bergh G and Arckens L. Recent advances in 2D electrophoresis: an array of possibilities. Expert Review of Proteomics. 2005; 2(2): 243–252.
14.    Zhou G, Li H, DeCamp D. 2D differential ingel electrophoresis for the identification of esophageal scans cell cancer-specific protein markers. Molecular and Cellular Proteomics. 2002;1(2):117–124.
15.    Gygi SP, Rist B, Gerber SA, Turecek F, Gelb MH, Aebersold R. Quantitative analysis of complex protein mixtures using isotope-coded affinity tags. Nature Biotechnology. 1999;17(10):994–999.
16.    Haqqani AS, Kelly JF, Stanimirovic DB. Quantitative protein profiling by mass spectrometry using isotopecoded affinity tags. Methods in Molecular Biology. 2008; 439: 225–240.
17.    Wu CC, MacCoss MJ, Howell KE, Matthews DE, Yates JR. Metabolic labeling of mammalian organisms with stable isotopes for quantitative proteomic analysis. Analytical Chemistry. 2005;76(17):4951–4959.
18.    Ross PL, Huang YN, Marchese JN. Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents. Molecular andCellular Proteomics. 2004; 3(12):1154–1169.
19.    William CS, Proteomics Technologies and Challenges. Geno. Prot. Bioinfo. 2007;5(2):77-85.
20.    Dubey H and Grover A. Current initiatives in proteomics research: the plant perspective. Current Science. 2001;80(2):262–269.
21.    Ye X, Luke B, Andresson T, Blonder J. 18O stable isotope labeling in MS-based proteomics. Briefings in Functional Genomics and Proteomics. 2009; 8(2):136–144.
22.    Chandramouli K and Qian PY. Proteomics: Challenges, Techniques and Possibilities to Overcome Biological Sample Complexity. Human Genomics and Proteomics. 2009. doi:10.4061/2009/239204
23.    Cristea IM, Gaskell SJ, Whetton AD. Proteomics Techniques and their application to Hematology. Blood. 2004;103(10):3624-3534. doi:10.1182/blood-2003-09-3295.
24.    Yates J. Recent technical advances in proteomics. F1000Research. 2019;351:1-8. https://doi.org/10.12688/f1000research.16987.1.
25.    Saha D, Tamrakar A. Protein Mass Spectrometry: Novel Approaches in Pharmaceutical Biotechnology. Asian J. Pharm. Ana. 2011;1(2):25-26.
26.    Yadav AR, Mohite SK. Recent Advances in Protein and Peptide Drug Delivery. Res. J. Pharma. Dosage Forms and Tech. 2020;12(3):205-212.
27.    Salve P, Kirtawade R, Gharge D, Dhabale P, Burade K. Proteomics: A Tool In Future. Research J. Pharmacology and Pharmacodynamics. 2009;1(3):99-103.
28.    Dubey RD, Ahmad AR, Paroha S, Gupta RK, Sahu PK, Verma S, Daharwal SJ, Prasad Reddy SLN. Mass Spectroscopy: A Versatile Analytical Technique. Research J. Science and Tech. 2011; 3(2): 55-64.
29.    K. Pradeep, R.L. Priyadarshini, K. Vijay. Peptide Microarray Production on a Solid Support and its Applications. Research J. Pharm. and Tech. 2012; 5(8):1015-1024.
30.    Babu VR, Syeda RN, Nivethithai P, Areefulla SH. Approaches and Challenges of Protein and Peptide Drug Delivery Systems. Research J. Pharm. and Tech. 2010; 3(2):379-384.
31.    Ramraje GR, Patil SD, Patil PH, Pawar AR. A Brief Review on: Separation Techniques Chromatography. Asian J. Pharm. Ana. 2020;10(4):231-238.
32.    Sharma S, Singh N, Ankalgi AD, Rana A, Ashawat M. A Laconic Review on Liquid Chromatography Mass Spectrometry (LC-MS) Based Proteomics Technology in Drug Discovery. Asian Journal of Pharmaceutical Research. 2021; 11(3):194-1.
33.    Patel VD, Patel MB, Anand IS, Patel CN, Bhatt PA. Toxicogenomics: A Review. Research J. Pharmacology and Pharmacodynamics. 2010;2(2):131-140.
34.    More S, Raje V, Phalke N, Lokhande S. Bioinformatics – An Emerging Field. Asian J. Res. Pharm. Sci. 2018;8(4):185-191.
35.    Dwivedi B, Mishra PK, Mishra SK. Quantitative Analysis of ATM, ATR and γ-H2AX as DNA Damage Sensor Proteins through Western Blotting. Research J. Science and Tech. 2014; 6(2): 91-94.

Recomonded Articles:

Author(s): Hiral A. Makadia, Ami Y. Bhatt, Ramesh B. Parmar, Ms. Jalpa S. Paun, H.M. Tank

DOI:         Access: Open Access Read More

Author(s): Neha Meshram, Mithlesh Ojha, Ajay Singh, Amit Alexander, Ajazuddin, Mukesh Sharma

DOI: 10.5958/2231-5691.2015.00009.X         Access: Open Access Read More

Author(s): Sandesh Narayan Somnache, Ajeet Madhukar Godbole, Pankaj Sadashiv Gajare, Sapna Kashyap

DOI: 10.5958/2231-5691.2016.00028.9         Access: Open Access Read More

Author(s): B.A. Bhairav, J.K. Bachhav, R.B. Saudagar

DOI: 10.5958/2231-5691.2016.00025.3         Access: Open Access Read More

Author(s): Sarin.a. Chavhan, Sushilkumar. A. Shinde, Sandip. B. Sapkal , Vinayak N. Shrikhande

DOI: 10.5958/2231-5691.2017.00019.3         Access: Open Access Read More

Author(s): Debarshi Kar Mahapatra, Ruchi S. Shivhare, Pranesh Kumar

DOI: 10.5958/2231-5691.2018.00002.3         Access: Open Access Read More

Author(s): Prabhu Venkatesh Moodbidri, Varadaraji Dhayanithi, Ganesh Belavadi Manjunathashastry, Hari Narayan Pati , Pardhasaradhi Vasireddy

DOI: 10.5958/2231-5691.2015.00027.1         Access: Open Access Read More

Author(s): A.B. Roge, P.S. Tarte, M.M. Kumare, G.R. Shendarkar, S.M. Vadvalkar

DOI:         Access: Open Access Read More

Author(s): Jude Chigozie Ikewuchi, Catherine Chidinma Ikewuchi and Mercy Onuekwuzu Ifeanacho

DOI:         Access: Open Access Read More

Author(s): Batra Neha, Jain Honey, Bairwa Ranjan, Bachwani Mukesh

DOI:         Access: Open Access Read More

Author(s): Aamir Nazir1, Neha Kumari, Nitan Bharti Gupta, Rajesh Gupta, Ashima Chandel, Abi C. Varghese

DOI: 10.52711/2231-5691.2023.00030         Access: Closed Access Read More

Asian Journal of Pharmaceutical Research (AJPRes.) is an international, peer-reviewed journal, devoted to pharmaceutical sciences. AJPRes. publishes Original Research Articles, Short Communications..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231–5691 


Recent Articles




Tags