Author(s): Sravani Koralla, Sathish Kumar Konidala, K Govinda Rao, Sheik Mobina Begum

Email(s): sravanikoralla@gmail.com

DOI: Not Available

Address: Sravani Koralla1*, Sathish Kumar Konidala2, K Govinda Rao1, Sheik Mobina Begum2
1Department of Pharmaceutical Chemistry, Aditya Pharmacy College, Surampalem, E.G., A.P., India-533347
2Department of Pharmaceutical Analysis and Quality Control, Aditya Pharmacy College, Surampalem, E.G., A.P., India-533347
*Corresponding Author

Published In:   Volume - 6,      Issue - 4,     Year - 2016


ABSTRACT:
A simple and accurate stability indicating RP-HPLC method for the simultaneous estimation of Ramipril and amlodipine besylate in pharmaceutical dosage form was developed and validated. The separation of selected drugs was done by using, X-Bridge C18 (150 x 4.6 mm, 5µ) column as stationary phase and a mixture of 5 mM Ammonium acetate(45): ACN(55): 0.1%formic acid(0.5) as mobile phase. Flow rate of mobile phase was maintained at 1 ml/min at ambient temperature throughout the experiment. Quantification was achieved with ultraviolet (DAD) detection at 240 nm. The retention times of Ramipril and amlodipine besylate were found as 5.653minutes and 6.944 minutes respectively. The detector response was linear in the concentration range of 4-12µg/ml for both drugs and the regression coefficients found as 0.9999 and 0.9998 for Ramipril and amlodipine respectively. This method was found free of any interference from any excipients. The percentage recovery values for Ramipril and amlodipine was found to be in the range of 98.83-101.09% and 98.40-100.13%. The Ramipril and amlodipine subjected to stress conditions including acidic, alkaline, oxidation, photolysis and thermal degradation. Ramipril and amlodipine besylate were more sensitive towards alkali and oxidative degradation. The developed method has been optimized and validated according to ICH guidelines and shown to be Specific, Sensitive, Precise, Accurate, Rugged and Robust. Hence, this method can be applied for routine quality control of Ramipril and amlodipine besylate in dosage forms as well as in bulk drug.


Cite this article:
Sravani Koralla, Sathish Kumar Konidala, K Govinda Rao, Sheik Mobina Begum. Stability Indicating RP-HPLC Method For Simultaneous Estimation of Ramipril and Amlodipine Besylate in Pharmaceutical Dosage Form. Asian J. Pharm. Res. 2016; 6(4): 242-249.


Recomonded Articles:

Author(s): Habeeb Ahmed, Mohammed Ehtesham, Nuha Rasheed , Abdul Saleem Mohammad

DOI: 10.5958/2231-5691.2017.00006.5         Access: Open Access Read More

Author(s): Sandesh Narayan Somnache, Ajeet Madhukar Godbole, Pankaj Sadashiv Gajare, Sapna Kashyap

DOI: 10.5958/2231-5691.2016.00028.9         Access: Open Access Read More

Author(s): Sheetal B. Gondkar, Shalaka P. Rasal, Ravindra B. Saudagar

DOI: 10.5958/2231-5691.2016.00027.7         Access: Open Access Read More

Author(s): Audumbar Mali, Santosh Jadhav, Rahul Gorad, Aamer Quazi, Ritesh Bath, Manojkumar Patil, Ashpak Tamboli

DOI: 10.5958/2231-5691.2016.00017.4         Access: Open Access Read More

Author(s): Nikita R. Nikam, Rohan R. Vakhariya, Dr. C. S. Magdum

DOI: 10.5958/2231-5691.2019.00018.2         Access: Closed Access Read More

Author(s): Vidya Raju*, Jasmine Joy Bell, Merlin. N. J, Shaiju S Dharan

DOI: 10.5958/2231-5691.2017.00037.5         Access: Open Access Read More

Author(s): B.A. Bhairav, J.K. Bachhav, R.B. Saudagar

DOI: 10.5958/2231-5691.2016.00025.3         Access: Open Access Read More

Author(s): Rutuja S. Shah, Rutuja R. Shah, Manoj M. Nitalikar, Chandrakant S. Magdum

DOI: 10.5958/2231-5691.2017.00024.7         Access: Open Access Read More

Author(s): Kasad Pinaz A, K.S. Muralikrishna

DOI:         Access: Open Access Read More

Author(s): Prabhu Venkatesh Moodbidri, Varadaraji Dhayanithi, Ganesh Belavadi Manjunathashastry, Hari Narayan Pati , Pardhasaradhi Vasireddy

DOI: 10.5958/2231-5691.2015.00027.1         Access: Open Access Read More

Author(s): Sufiyan Ahmad, Ansari Sajjad, Md. Rageeb Md. Usman, Mohammed Imran, Rashid Akhtar

DOI: 10.5958/2231-5691.2017.00022.3         Access: Open Access Read More

Author(s): Subhashis Debnath, C. Navya Yadav, N. Nowjiya, M. Prabhavathi, A. SaiKumar, P. Sai Krishna, M. Niranjan Babu

DOI: 10.5958/2231-5691.2019.00009.1         Access: Closed Access Read More

Asian Journal of Pharmaceutical Research (AJPRes.) is an international, peer-reviewed journal, devoted to pharmaceutical sciences. AJPRes. publishes Original Research Articles, Short Communications..... Read more >>>

RNI: Not Available                     
DOI: 10.5958/2231–5691 


Recent Articles




Tags