Author(s): Reenu Joseph, Shamina Azeez, Chempakam Bhageerathy

Email(s): reenujoseph09@gmail.com

DOI: 10.52711/2231-5691.2023.00041   

Address: Reenu Joseph1*, Shamina Azeez2, Chempakam Bhageerathy1
1ICAR-Indian Institute of Spices Research, Kozhikode – 673012, Kerala, India.
2ICAR-Indian Institute of Horticultural Research, Bengaluru – 560089, Karnataka, India.
*Corresponding Author

Published In:   Volume - 13,      Issue - 4,     Year - 2023


ABSTRACT:
The objective of the present study is to quantify and compare in vitro cytotoxic properties of crude extracts sequentially extracted in solvents from the least polar hexane to the most polar water (hexane, petroleum ether, benzene, chloroform, ethyl acetate, methanol and water) from fresh and dry rhizomes of black turmeric on human cancer cell lines and further identification of phenolic acids and flavonoids by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the active extracts. Of the extracts analyzed, chloroform and ethyl acetate extracts exhibited significant cytotoxicity on the tested cells. LC-MS/MS analysis of the active extracts (chloroform and ethyl acetate) revealed the presence of 14 phenolic acids and 9 flavonoids for the first time in the rhizomes of C. caesia, phenolic acids present in high concentration being gallic and vanillic acids in chloroform extracts, vanillic and protocatechuic acids in ethyl acetate extracts and catechin being the most abundant flavonoid. The active ingredients gallic acid, vanillic acid, protocatechuic acid and catechin present in these extracts may act as lead compounds for the development of new drugs against cancer.


Cite this article:
Reenu Joseph, Shamina Azeez, Chempakam Bhageerathy. Anticancer effect of sequential extracts from Curcuma caesia rhizomes on human cancer cell lines and characterization of selected polyphenols in active extracts by LC-MS/MS. Asian Journal of Pharmaceutical Research. 2023; 13(4):219-6. doi: 10.52711/2231-5691.2023.00041

Cite(Electronic):
Reenu Joseph, Shamina Azeez, Chempakam Bhageerathy. Anticancer effect of sequential extracts from Curcuma caesia rhizomes on human cancer cell lines and characterization of selected polyphenols in active extracts by LC-MS/MS. Asian Journal of Pharmaceutical Research. 2023; 13(4):219-6. doi: 10.52711/2231-5691.2023.00041   Available on: https://asianjpr.com/AbstractView.aspx?PID=2023-13-4-2


REFERENCES:
1.    Gupta P, Khan MY, Verma VK and Pathak A. Beating Cancer with Natural Plant Sources. Asian Journal of Pharmacy and Technology. 2013; 3(2):39-44
2.    Ghouse MS. An overview on plant derived anticancer drugs. Research Journal of Pharmacognosy and Phytochemistry. 2020; 12(4):235-244.doi.org/10.5958/0975-4385.2020.00040.0
3.    Kinghorn AD, Fransworth NR, Soejarto DD, Cordell GA, Swanson SM, Pezzuto JM, Wani MC, Wall ME, Oberlies NH, Kroll DJ, Kramer RA, Rose WC, Vite GD, Fairchild CR, Peterson RW and Wild R. Novel strategies for the discovery of plant derived anticancer agents. Pharmaceutical Biology. 2003; 41 Suppl 1:53-67. doi: 10.1007/978-1-4615-2610-0_4
4.    Sasikumar B. Genetic resource of Curcuma: diversity, characterization and utilization. Plant Genetic Resoures-C 2005; 3:230-251. doi.org/10.1079/PGR200574
5.    Banerjee A and Nigam SS. Antifungal activities of the essential oil of Curcuma caesia Roxb. Indian Journal of Medical Research. 1976; 64(9):1318-1321.
6.    Arulmozhi DK, Sridhar N, Veeranjaneyulu A and Arora SK. Preliminary mechanistic studies on the smooth muscle relaxant effect of hydroalcoholic extract of Curcuma caesia. Journal of Herbal Pharmacotherapy. 2006; 6:117-124.doi.org/10.1080/J157v06n03_06
7.    Karmakar I, Saha P, Sarkar N, Bhattacharya S and Haldar PK. Neuropharmacological assessment of Curcuma caesia rhizome in experimental animal models. Oriental Pharmacy and Experimental Medicine. 2011; 11:251-255.doi.org/10.1007/s13596-011-0032-4
8.    Paliwal P, Pancholi SS and Patel RK. Comparative evaluation of some plant extracts on bronchoconstriction in experimental animals. Asian Journal of Pharmacy and Life Sciences 2011; 1(1).
9.    Krishnaraj M, Manibhushanrao K and Mathivanan N. A comparative study of phenol content and antioxidant activity between non-conventional Curcuma caesia Roxb. and Curcuma amada Roxb. International Journal of Plant Production. 2010; 4(3):169-174.doi.org/10.22069/IJPP.2012.693
10.    Mangla M, Mohd. S, Jain J and Kashyap M. In vitro evaluation of antioxidant activity of Curcuma caesia Roxb. International Journal of Pharmaceutical Sciences and Research. 2010; 1:98-102.doi.org/10.13040/IJPSR.0975-8232.1(9-S).98-02
11.    Sawant SB, Bihani G, Mohod S and Bodhankar S. Evaluation of analgesic and anti-inflammatory activity of methanolic extract of Curcuma caesia Roxb. rhizomes in laboratory animals.  International Journal of Pharmacy and Pharmaceutical Sciences. 2014; 6(2):243-247.
12.    Karmakar I, Dolai N, Suresh Kumar RB, Kar B, Roy SN and Haldar PK. Antitumor activity and antioxidant property of Curcuma caesia against Ehrlich's ascites carcinoma bearing mice. Pharmaceutical Biology. 2013; 51(6):753-759.doi.org/10.3109/13880209.2013.764538
13.    Liu Y, Roy SS, Nebie RHC and Nair MG. Functional food quality of Curcuma caesia, Curcuma zedoaria and Curcuma aeruginosa endemic to northeastern India. Plant Foods for Human Nutrition. 2013; 68:72-77.doi.org/10.1007/s11130-013-0333-5
14.    Freshney IR. Culture of animal cells-a manual of basic technique. Wiley-Liss, Inc, Fifth Edition 2007.
15.    Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods. 1983; 65(1-2):55-63.doi.org/10.1016/0022-1759(83)90303-4
16.    Neeta and Dureja H. Reverse phase high-performance liquid chromatographic estimation and in vitro cytotoxicity of boswellic acids on A-375 melanoma cancer cell lines. Asian Journal of Pharmaceutical Analysis. 2018; 8(1):13-19. doi.org/10.5958/2231-5675.2018.00003.0
17.    Reenu J, Azeez S and Bhageerathy C. In vitro antioxidant potential in sequential extracts of Curcuma caesia Roxb. rhizomes. Indian Journal of Pharmaceutical Sciences. 2015; 77(1):41-48.doi.org/10.4103%2F0250-474x.151596
18.    Rates SM. Plants as source of drugs. Toxicon. 2001; 39(5):603-613.doi.org/10.1016/s0041-0101(00)00154-9
19.    Kamuhabwa A, Nshimo C and Witte PD. Cytotoxicity of some medicinal plant extracts used in Tanzanian traditional medicine. Journal of Ethnopharmacology. 2010; 70:143-149.doi.org/10.1016/s0378-8741(99)00161-0
20.    Tokarska-Schlattner M, Wallimann T and Schlattner U. Alterations in myocardial energy metabolism induced by the anti-cancer drug doxorubicin. Comptes Rendus Biologies. 2006; 329:657-668.doi.org/10.1016/j.crvi.2005.08.007
21.    Sagar K, Aneesha S, Uppin P, Gowthami. Phytochemical studies and quantification of total content of phenols, tannins and flavonoids in selected endangered plant species. Research Journal of Pharmacognosy and Phytochemistry. 2018; 10(4): 277-281.doi.org/10.5958/0975-4385.2018.00044.4
22.    Tapas A, Sakarkar D and Kakde R. The chemistry and biology of bioflavonoids. Research Journal of Pharmacy and Technology. 2008; 1(3): 132-143.
23.    Geran RI, Greenberg NH, McDonald MM, Schumacher AM and Abbott BJ. Protocols for screening chemical agents and natural products against animal tumor and other biological systems. Cancer Chemotherapy Reports. 1972; 3:17-19.
24.    Lo C, Lai TY, Yang JH, Yang JS, Ma YS, Weng SW, Chen YY, Lin JG and Chung JG. Gallic acid induces apoptosis in A375. S2 human melanoma cells through caspase-dependent and independent pathways. International Journal of Oncology. 2010; 37(2):377.-385doi.org/10.3892/ijo_00000686
25.    Inoue M, Suzuki R, Koide T, Sakaguchi N, Ogihara Y and Yabu Y. Antioxidant, gallic acid, induces apoptosis in HL-60RG cells. Biochemical and Biophysical Research Communications. 1994; 204:898-904.doi.org/10.1006/bbrc.1994.2544
26.    Kawada M, Ohno Y, Ri Y, Ikoma T, Yuugetu H, Asai T, Watanabe M, Yasuda N, Akao S, Takemura G, Minatoguchi S, Gotoh K, Fugiwara H and Fukuda K. Anti-tumor effect of gallic acid on LL-2 lung cancer cells transplanted in mice. Anti-cancer Drugs. 2001; 12:847-852.doi.org/10.1097/00001813-200111000-00009
27.    Lirdprapamongkol K, Kramb JP, Suthiphongchai T, Surarit R, Srisomsap C, Dannhardt G and Svasti J. Vanillin suppresses metastatic potential of human cancer cells through PI3K inhibition and decreases angiogenesis in vivo. Journal of Agricultural and Food Chemistry. 2009; 57(8): 3055-3063.doi.org/10.1021/jf803366f
28.    Yin MC, Lin CC, Wu HC, Tsao SM and Hsu CK. Apoptotic effects of protocatechuic acid in human breast, lung, liver, cervix, and prostate cancer cells: potential mechanisms of action. Journal of Agricultural and Food Chemistry. 2009; 57(14):6468-6473.doi.org/10.1021/jf9004466
29.    Larsen CA, Bisson WH and Dashwood RH. Tea catechins inhibit hepatocyte growth factor receptor (MET kinase) activity in human colon cancer cells: kinetic and molecular docking studies. Journal of Medicinal Chemistry. 2009; 52:6543-6545.doi.org/10.1021/jm901330e
30.    Manikandan R, Beulaja M, Arulvasu C, Sellamuthu S, Dinesh D, Prabhu D, Babu G, Vaseeharan B and Prabhu NM. Synergistic anticancer activity of curcumin and catechin: an in vitro study using human cancer cell lines. Microscopy Research and Technique. 2012; 75:112-116.doi.org/10.1002/jemt.21032
31.    Manju V and Nalini N. Protective role of luteolin in 1,2-dimethylhydrazine induced experimental colon carcinogenesis. Cell Biochemistry and Function. 2007; 25:189-194.doi.org/10.1002/cbf.1305
32.    Sivagami G, Vinothkumar R, Preethy CP, Riyasdeen A, Akbarsha MA, Menon VP and Nalini N. Role of hesperetin (a natural flavonoid) and its analogue on apoptosis in HT-29 human colon adenocarcinoma cell line: a comparative study. Food and Chemical Toxicology. 2012; 50:660-671.doi.org/10.1016/j.fct.2011.11.038


Recomonded Articles:

Author(s): AK Meena, MM Rao, RP Meena, P Panda, Renu

DOI:         Access: Open Access Read More

Author(s): Mohite M S, Shelar P A, Raje V N., Babar S. J., Sapkal R. K.

DOI:         Access: Open Access Read More

Author(s): Neha Meshram, Mithlesh Ojha, Ajay Singh, Amit Alexander, Ajazuddin, Mukesh Sharma

DOI: 10.5958/2231-5691.2015.00009.X         Access: Open Access Read More

Author(s): N. S. Patil, K. B. Patil, M. R. Patil, R. A. Ahirrao

DOI: 10.5958/2231-5691.2018.00037.0         Access: Open Access Read More

Author(s): R. Sathish, P. Sravan Kumar, K. Natarajan, N. Sridhar

DOI:         Access: Open Access Read More

Author(s): Batra Neha, Jain Honey, Bairwa Ranjan, Bachwani Mukesh

DOI:         Access: Open Access Read More

Author(s): Ganesh Akula, Rangu Nirmala, CH. Shanthipriya, S. Rohini Reddy, A. Jaswanth

DOI: 10.5958/2231-5691.2017.00010.7         Access: Open Access Read More

Author(s): Srivastav Sanchit, Luhach Kanishk, Shukla Chhama, Yadav Rajender

DOI:         Access: Open Access Read More

Author(s): Farha Fatma, Anil Kumar

DOI: 10.52711/2231-5691.2021.00045         Access: Open Access Read More

Author(s): Elumalai A., Prakash Yoganandam G.

DOI:         Access: Open Access Read More

Author(s): Praveen Kumar Uppala, Murali Krishna. B, K. Atchuta Kumar, Vinay Ramji

DOI: 10.5958/2231-5691.2016.00022.8         Access: Open Access Read More

Author(s): Nitin Mahurkar, Sayeed ul hasan S.M

DOI:         Access: Open Access Read More

Author(s): Sonali P. Mahaparale, Reshma S. Kore

DOI: 10.5958/2231-5691.2019.00029.7         Access: Open Access Read More

Author(s): Praveen Radhakrishnan, Shinu Chacko

DOI: 10.5958/2231-5691.2018.00032.1         Access: Open Access Read More

Author(s): Nandhini S, Radha R , Vadivu R

DOI: 10.5958/2231-5691.2016.00019.8         Access: Open Access Read More


Recent Articles




Tags