Author(s):
Thamizharasi Suresh, Shankari Vellaisamy, Parvatha Raja Kumaran V S, Chetan Ashok
Email(s):
mstarasi@gmail.com
DOI:
10.52711/2231-5691.2024.00004 
Address:
Thamizharasi Suresh1*, Shankari Vellaisamy2, Parvatha Raja Kumaran V S3, Chetan Ashok4
1Associate Professor, Department of Pharmacognosy, K.K. College of Pharmacy, Chennai, Tamil Nadu.
2Associate Professor, Department of Pharmacology, K.K. College of Pharmacy, Chennai, Tamil Nadu.
3Saveetha College of Pharmacy, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu.
4Department of Pharmacology, Sri Ramachandra Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu.
*Corresponding Author
Published In:
Volume - 14,
Issue - 1,
Year - 2024
ABSTRACT:
Raphanus sativus (commonly called as Radish) belonging to family Brassicaceae which is a multipurpose plant with high attributes and potential. The study is aimed to evaluate the Pharmacognostic and to reveal the phytochemical profile of Raphanus sativus which serve as authentication and quality control for the crude drug. Microscopical studies and physiochemical parameters like ash value, extractive value, loss on drying and determination of crude fiber content were observed. Preliminary phytochemical screening was performed to identify the various phytoconstituents present in the crude drug. Macroscopical and phytochemical characters such as Fluorescence analysis and Thin Layer Chromatography were studied for the presence of secondary metabolites and High Performance Thin Layer Chromatography fingerprints were studied. Microscopic analysis and phytochemical evaluation provides sufficient data for characterize the drug. Preliminary Phytochemical investigation of Raphanus sativus were performed in various extraction solvents such as Ethanol, Chloroform and methanol and revealed the presence of alkaloids, carbohydrates, flavonoids, phytosterols, Steroids, Tannins and fixed oils. The study concluded that pharmacognostical evaluation data provided will be helpful in characterization of the crude drug and also leads in the development of the pharmaceuticals and research.
Cite this article:
Thamizharasi Suresh, Shankari Vellaisamy, Parvatha Raja Kumaran V S, Chetan Ashok. Pharmacognostical and Phytochemical Screening of Leaves of Raphanus sativus Linn. Asian Journal of Pharmaceutical Research. 2024; 14(1):25-2. doi: 10.52711/2231-5691.2024.00004
Cite(Electronic):
Thamizharasi Suresh, Shankari Vellaisamy, Parvatha Raja Kumaran V S, Chetan Ashok. Pharmacognostical and Phytochemical Screening of Leaves of Raphanus sativus Linn. Asian Journal of Pharmaceutical Research. 2024; 14(1):25-2. doi: 10.52711/2231-5691.2024.00004 Available on: https://asianjpr.com/AbstractView.aspx?PID=2024-14-1-4
REFERENCE:
1. Craig WJ. Health-promoting properties of common herbs. The American Journal of Clinical Nutrition. 1999; 70(3): 491s-9s.
2. Tripathi KD. Essentials of Medicinal Pharmacology. Cancer natural ayurvedic cure/ Cancer Remedies, Jaypee Publications, 19th. 2008; 769-782.
3. Achrya, Deepak, Anshu S. Indigenous herbal medicines; Tribal Formulation and Traditional Herbal Practices, Aavishkar Publishers Jaipur, India. 2008; 440.
4. Tapsell LC, Hemphill I, Cobiac L, Patch CS, Sullivan DR, Fenech M, Roodenrys S, Keogh JB, Clifton PM, Williams PG, Fazio VA, Inge KE. Health benefits of herbs and spices: the past, the present, the future. Med J Aust. 2006; 185(S4): S1-S24. https://doi.org/10.5694/j.1326-5377.2006.tb00548.x
5. Talalay P, Talalay P. The importance of using scientific principles in the development of medicinal agents from plants. Academic Medicine. 2001; 76(3): 238-47.
6. Elvin-Lewis M. Should we be concerned about herbal remedies. Journal of Ethnopharmacology. 2001; 75(2-3): 141-64. https://doi.org/10.1016/S0378-8741(00)00394-9
7. Sing VK, Govil GS. Recent progress in medicinal plants: ethnomedicine and pharmacognosy. Texas: SCI Tech Publishing LIC. 1985; 106
8. Wallis TE. Text book of Pharmacognosy, CBS Publishers and Distributers, 5th Edition. 1993; 1-6.
9. Vinod D Rangari. Text book of Pharmacognosy and Phytochemistry, Nishad Deshmukh for Career Publications, Maharashtra, 1986; 1-8.
10. Namrata S, Jain NK, Pushpendra K, Navneet G, Pathak AK, Mehta SC. In vitro antioxidant activity of Calotropis gigantea hydroalcohlic leaves extract. Der Pharmacia Lettre. 2010; 2(3): 95-100.
11. Cardoso-Ugarte GA, Sosa-Morales ME. Essential oils from herbs and spices as natural antioxidants: Diversity of promising food applications in the past decade. Food Reviews International. 2022 ; 38(sup1): 403-33. https://doi.org/10.1080/87559129.2021.1872084
12. Pereira AS, Banegas-Luna AJ, Peña-García J, Pérez-Sánchez H, Apostolides Z. Evaluation of the anti-diabetic activity of some common herbs and spices: Providing new insights with inverse virtual screening. Molecules. 2019; 24(22): 4030. https://doi.org/10.3390/molecules24224030
13. Manivannan A, Kim JH, Kim DS, Lee ES, Lee HE. Deciphering the nutraceutical potential of Raphanus sativus—a comprehensive overview. Nutrients. 2019; 11(2): 402. https://doi.org/10.3390/nu11020402
14. Gamba M, Asllanaj E, Raguindin PF, Glisic M, Franco OH, Minder B, Bussler W, Metzger B, Kern H, Muka T. Nutritional and phytochemical characterization of radish (Raphanus sativus): A systematic review. Trends in Food Science & Technology. 2021; 113: 205-18. https://doi.org/10.1016/j.tifs.2021.04.045
15. Sass JE. Elements of botanical microtechnique. Elements of Botanical Microtechnique. 1940; 225-233.
16. Aher N., Chaudhari S., Zalte A. Morphological and microscopical studies and Phytochemical analysis of Markhamia falcata (Seem). Research J. Pharm. and Tech. 2020; 13(3): 1117-1120. doi: 10.5958/0974-360X.2020.00205.X
17. Johansen DA. Plant microtechnique, Mc Grew Hill Book and Co, New York, 1940; 523-525.
18. Biren N shah, Nayak BS. Text book of Experimental Pharmacognosy, S. Vikas and Co, Jalandhar, 225-233.
19. Chandupatla Rahul Reddy, Kanakam Vijayabhaskar, Madugula Srikanth, Pingali Srinivasa Rao. Morphology and Microscopical Studies of Leaves, Stem and Roots of Trianthema portulacastrum Linn. Tella galijeru (Aizoaceae). Res. J. Pharmacology & Pharmacodynamics. 2019; 11(4): 132-136. doi: 10.5958/2321-5836.2019.00023.5
20. Khandelwal KR, Kokate CK. Text Book of Practical Pharmacognosy, Nirali Prakashan, 2nd edition, New Delhi, 1995; 9-30, 146-150.
21. Anonymous. Quality Control for Medicinal Plant Materials, WHO, Geneva, Indian edition, 2004; 28-30.
22. Mukarjee PK. Quality control of herbal drugs and approach to evaluation of botanicals, Business horizons pharmaceutical publishers, 1st edition, New Delhi, 2002; 186-191, 380-421.
23. Kavitha M, Dr. Vadivu R, Dr. Radha R. Establishment of Pharmacognostical Standards for the Bark of Sterculia foetida linn. Res. J. Pharmacognosy and Phytochem. 2016; 8(2): 75-80. doi: 10.5958/0975-4385.2016.00014.5
24. P. Selvakumar, Devi Kaniakumari, V. Loganathan. Physicochemical Analysis of Alocasia sanderiana W. Bull. Asian J. Pharm. Ana. 2016; 6(1): 31-34.
25. A. Sureka, R. Dhanalakshmi, C. Mary Sharmila, R. Chithra Devi. Comparative Analysis on Significance of Purification Process of Monosilai in Siddha medicine using Modern Instrumental Techniques: Standardization Approach. Asian J. Pharm. Ana. 2019; 9(2): 99-104. doi: 10.5958/2231-5675.2019.00019.X
26. Kokate CK. Text book of Practical Pharmacognosy, Vallabh Prakashan, 3rd edition, New Delhi, 1994; 107-111, 115-125.
27. Kokate CK. Text Book of Practical Pharmacognosy, Vallabh Prakashan, 4th Edition, New Delhi, 123-124.
28. Rangarajan Narasimhan, Sathiyamoorthy. M. Phytochemical Screening and Antioxidant Studies in the Pulp Extracts of Cucurbita maxima. Asian J. Pharm. Res. 2016; 6(1): 1-4. doi: 10.5958/2231-5691.2016.00001.0
29. K. P. Jaiganesh, B. Sreedharren, G. Arunachalam, R. Nirmala, R. Nepolean. Pharmacognostical and Antimicrobial investigation of Jatropha curcas, Linn., Leaf. Asian J. Res. Pharm. Sci. 2013; 3(4): 195-199.
30. Ragya Eslavath, V. Harikrishna, N. Kosuru, Goli Venkateshwarlu, Manoranjan Sabat, Kanakaiah K.. Phytochemical Screening and TLC, UV-Spectrophotometer Study of Bougainvillea glabra. Asian J. Pharm. Ana. 2013; 3(3): 83-85.
31. Suresh G. Killedar, Harinath N. More. Screening of Antimicrobial Potential and Phytoconsituents for Different Extracts of Memecylon umbellatum Burm Inflorescences. Asian J. Pharm. Res. 2011;1(4): 114-118.
32. Harborne AJ. Phytochemical methods a guide to modern techniques of plant analysis. Chapman and Hall, 3rd edition, United Kingdom. 1998; 60-65.
33. Raman N. Text book of Phytochemical Techniques, New India publishing agency – CBS Publishers, 5th edition, New Delhi; 19-24.
34. Rajabudeen E, Saravana Ganthi A, M Padma Sorna Subramanian. Pharmacognostical Studies on Tephrosia villosa (L.) Pers. (Fabaceae). Res. J. Pharmacognosy & Phytochem. 2014; 6(4): 190-194.
35. Dharamveer, Bharat Mishra, H.H. Siddiqui. Pharmacognostical and phytochemical studies on Anacardium occidentale Linn. leaves. Research J. Pharm. and Tech. 2013; 6(1): 75-79.
36. Deepak M, Sulaiman CT, Athulya M, Indira Balachandran. Phytochemical and Chromatographic studies of two Ayurvedic source plants of Somalata, Cynanchum viminale and Ceropegia juncea. Asian Journal of Pharmaceutical Research. 2021; 11(3): 156-2. doi: 10.52711/2231-5691.2021.00029
37. Sreedharren B, Jaiganesh KP, Kannappan N, Sulochna N. Pharmacognostic studies on Plectranthus amboinicus Lour. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2010; 1(4): 413-24. http://rjpbcs.com/pdf/2010_1(4)/[46].pdf
38. Abragam DA, Kalidass C, Mohan VR. Pharmacognostical Investigation of Hedyotis puberula (G. Don) Arn. Asian Journal Exp. Boil. Sci, 2010; 1(2): 373-377.
39. Schibli A, Reich E. Modern TLC: a key technique for identification and quality control of botanicals and dietary supplements. JPC-Journal of Planar Chromatography-Modern TLC. 2005; 18(101): 34-8.
40. Kokate CK, Purohit AP, Gokhale SB. Practical Pharmacognosy, Niraliprakashan, 23rd edition, New Delhi, 2005; 8:1-8.23.
41. Wagner H, Bladt S. Plant drug analysis: a thin layer chromatography atlas. Springer Science & Business Media, 2nd Edition, 1996; 195.
42. Manimaran S, Subburaju T, Raja SS, Alok S, VALBHAV G, Chinnasamy K, Nanjan MJ, Suresh B. Comparative study of gallic acid content in marketed siddha formulations of triphala chooranam and Tablets by HPTLC. Indian Drugs. 2003; 40(9): 532-534.
43. Anonymous. Indian Herbal Pharmacopeia, Indian drug manufacturer association, Mumbai. 2002; 179-180.
44. Bhavana Srivastava, Himanshu Sharma, Vikas Chandra Sharma, S.C. Verma, A.D. Jadhav. Pterocarpus marsupium, physicochemical analysis, phytochemical analysis, HPTLC profile. Res. J. Pharmacognosy and Phytochem. 2016; 8(2): 53-59. doi: 10.5958/0975-4385.2016.00010.8